Invitrogen™ Low Density Lipoprotein from Human Plasma, BODIPY™ FL ... PDF Membrane Fluidity and Lipid Order in Ternary Giant Unilamellar Vesicles ... Invitrogen™ BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene) Real-time quantitative RT-PCR analyses were performed with a Stratagene Mx3000p system and Mesa Green qPCR MasterMix Plus for SYBR® (RT-SY2X-03+WOULR . Many fluorescent lipid probes tend to loop back to the membrane interface when attached to a lipid acyl chain rather than embedding deeply into the bilayer. Two-photon time-lapse microscopy of BODIPY-cholesterol reveals ... We also show that over-expression of human Patched in the yeast S. cerevisiae results in a significant boost of BODIPY-cholesterol efflux. Apolipoprotein E gene polymorphism influences aggressive behavior in ... For the BODIPY-cholesterol assay, ram spermatozoa were labelled with BODIPY-cholesterol and prepared for in vitro capacitation as previously described (Bernecic et al. Labelingmedium wasprepared throughmixing unlabeled cholesterol (0.025mM),BODIPY- cholesterol (0.1mM) andcyclo- A, structure of BODIPY-cholesterol with the fluorophore (green, with light green underlay to highlight the fluorescent group) at carbon 24 of the sterol side chain. PMP22 Regulates Cholesterol Trafficking and ABCA1-Mediated Cholesterol ... Bodipy Lipid Droplet Staining Protocol This probe closely mimics the membrane partitioning and trafficking of cholesterol. Which is a unique fatty acid composition of the er and preliminary work describes differential display low cholesterol efflux from fission yeast. Two-photon time-lapse microscopy of BODIPY-cholesterol reveals ...
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