CF®550R Dye - Biotium CF®430 and CF®440 are much more photostable than spectrally similar dyes, making them suitable for imaging applications using confocal microscopy with 405 nm excitation. atto 550 flow cytometry channel Quantitative Comparison of Long-wavelength Alexa Fluor Dyes to Cy Dyes: Fluorescence of the Dyes and Their Bioconjugates The dye is moderately hydrophilic. 5 reviews. flow cytometry ATTO 550 is a novel fluorescent label related to the well-known dyes Rhodamine 6G and Rhodamine B. 690. It is commonly conjugated to antibodies and proteins for cellular imaging applications. Phone: +1 877 302 8632 Fax: +1 888 205 9894 (Toll-free) E-Mail: [email protected] None available. FLUOROPHORE TABLE - Abcam The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to … During step 7, prepare the conjugated antibody solution (to be used in step 11), by adding the desired antibodies to the CODEX FF staining solution to a final volume of 100 μl. Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Fluorescence was analyzed on a BD FACSCalibur flow cytometer in the FL4 channel. Lab on a Chip PAPER Cite this: Lab Chip,2017,17,830 Received 20th October 2016, Accepted 18th January 2017 DOI: 10.1039/c6lc01302c rsc.li/loc A nano flow cytometer for single lipid vesicle As shown in Fig. BD FACS Aria II Fluorochromes PMT A PMT B Hoechst (red for SP) (675/50 BP) BUV 395 (379/28 BP) Hoechst (blue for SP) (450/50 BP) Hoechst for viability (450/50 BP) Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 550 ...
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